Journal: International Journal of Molecular Sciences

Article Title: Reprogramming Chromosome Ends by Functional Histone Acetylation

doi: 10.3390/ijms25073898

Figure Lengend Snippet: ZSCAN4 binds to telomeres, leading to increased histone H3 acetylation. The “% Input” values represent the enrichment of ZSCAN4, Alu, or histone acetylation on a specific region from each sample: without (Dox−) or with ZSCAN4 induction (Dox+): ( A ) ZSCAN4 interaction with the telomeres was assessed by ZSCAN4-ChIP, followed by telomere qPCR and ( B ) Alu qPCR as control. ( C ) The effect of ZSCAN4 induction for 48 h on telomere-histone H3 acetylation was assessed by ChIP using H3K14ac or H3K18ac antibodies, followed by telomere qPCR. ( D ) Alu repeats were used as controls. Data are shown as mean ± S.E.M. * p < 0.05. **** p < 0.0001. ns—not significant.

Article Snippet: Antibodies used for ChIP: Anti-ZSCAN4 (10 μg) (Origene); anti-H3K14ac, H3K18ac, or H3K27ac (4 μg) (Cell signaling, Danvers, MA) and the provided anti-RNA Polymerase II (1 μg) and a Rabbit IgG (10 μg) were used as controls.

Techniques: Control

Journal: Cell

Article Title: SINGLE-CELL CHROMATIN MODIFICATION PROFILING REVEALS INCREASED EPIGENETIC VARIATIONS WITH AGING

doi: 10.1016/j.cell.2018.03.079

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal anti-acetyl-Histone H3 (Lys56) (clone 12.1) , Active Motif , 61061.

Techniques: Formulation, Recombinant, Red Blood Cell Lysis, Electron Microscopy, Software, Labeling, Blocking Assay